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Data sets used for training

The positive training set (experimentally verified chloroplast proteins) that was used for training LOCALIZER can be found here: Chloroplast transit peptides FASTA file.
The negative training set (experimentally verified non-chloroplast proteins) that was used for training LOCALIZER can be found here: Non-chloroplast proteins FASTA file.

The positive training set (experimentally verified mitochondrial proteins) that was used for training LOCALIZER can be found here: Mitochondrial transit peptides FASTA file.
The negative training set (experimentally verified non-mitochondrial proteins) that was used for training LOCALIZER can be found here: Non-mitochondrial proteins FASTA file.

The list of NLSs that is used for LOCALIZER can be found here: List of NLSs.

Data sets used for testing

The cropPal set of chloroplast proteins used in the evaluation: Chloroplast crop proteins FASTA file.
The cropPal set of mitochondrial proteins used in the evaluation: Mitochondrial crop proteins FASTA file.
The cropPal set of dual-targeted chloroplast/mitochondrial proteins used in the evaluation: Dual-targeted chloroplast/mitochondrial crop proteins FASTA file.
The cropPal set of nuclear proteins used in the evaluation: Nuclear crop proteins FASTA file.
The cropPal set of peroxisome proteins used in the evaluation: Peroxisome crop proteins FASTA file.
The cropPal set of vacuole proteins used in the evaluation: Vacuole crop proteins FASTA file.
The cropPal set of plasma membrane proteins used in the evaluation: Plasma membrane crop proteins FASTA file.
The cropPal set of ER proteins used in the evaluation: ER crop proteins FASTA file.
The cropPal set of cytosolic proteins used in the evaluation: Cytosolic crop proteins FASTA file.

The UniProt set of chloroplast proteins used in the evaluation: Chloroplast proteins FASTA file.
The UniProt set of mitochondrial proteins used in the evaluation: Mitochondrial proteins FASTA file.
The UniProt set of secreted proteins used in the evaluation: Secreted proteins FASTA file.
The UniProt set of nuclear proteins used in the evaluation: Nuclear proteins FASTA file.
The UniProt set of peroxisome proteins used in the evaluation: Peroxisome proteins FASTA file.
The UniProt set of vacuole proteins used in the evaluation: Vacuole proteins FASTA file.
The UniProt set of cytoplasmic proteins used in the evaluation: Cytoplasmic proteins FASTA file.
The UniProt set of ER proteins used in the evaluation: ER proteins FASTA file.

The set of chloroplast effectors used in the evaluation: Chloroplast effectors FASTA file.
The set of nuclear effectors used in the evaluation: Nuclear effectors FASTA file.
The set of HaRxLR mature effector sequences used in the evaluation: HaRxLR mature effectors FASTA file.
The negative test set of rust effector candidates with no determined localization (references here, here and here) used in the evaluation: Rust candidate effectors FASTA file.
The combined set of 107 fungal and oomycete effectors used in the evaluation: 107 effectors FASTA file.
The set of 69 fungal effectors used in the evaluation: Fungal effectors FASTA file.
The set of 51 oomycete effectors used in the evaluation: Oomycete effectors FASTA file.
The set of 358 RxLR effectors from a HMM model used in the evaluation: RxLR effectors FASTA file.

Detailed results from data presented in Table 4 in the LOCALIZER paper

The predicted Agaricus bisporus var bisporus secretome used in the evaluation: FASTA file
The predicted Agaricus bisporus var bisporus EffectorP candidates from the secretome: FASTA file
The predicted Agaricus bisporus var bisporus EffectorP candidates from the secretome run through LOCALIZER: ZIP file

The predicted Amanita muscaria secretome used in the evaluation: FASTA file
The predicted Amanita muscaria EffectorP candidates from the secretome: FASTA file
The predicted Amanita muscaria EffectorP candidates from the secretome run through LOCALIZER: ZIP file

The predicted Ashbya gossypii secretome used in the evaluation: FASTA file
The predicted Ashbya gossypii EffectorP candidates from the secretome: FASTA file
The predicted Ashbya gossypii EffectorP candidates from the secretome run through LOCALIZER: ZIP file

The predicted Aspergillus flavus secretome used in the evaluation: FASTA file
The predicted Aspergillus flavus EffectorP candidates from the secretome: FASTA file
The predicted Aspergillus flavus EffectorP candidates from the secretome run through LOCALIZER: ZIP file

The predicted Aspergillus niger secretome used in the evaluation: FASTA file
The predicted Aspergillus niger EffectorP candidates from the secretome: FASTA file
The predicted Aspergillus niger EffectorP candidates from the secretome run through LOCALIZER: ZIP file

The predicted Aspergillus oryzae secretome used in the evaluation: FASTA file
The predicted Aspergillus oryzae EffectorP candidates from the secretome: FASTA file
The predicted Aspergillus oryzae EffectorP candidates from the secretome run through LOCALIZER: ZIP file

The predicted Batrachochytrium dendrobatidis secretome used in the evaluation: FASTA file
The predicted Batrachochytrium dendrobatidis EffectorP candidates from the secretome: FASTA file
The predicted Batrachochytrium dendrobatidis EffectorP candidates from the secretome run through LOCALIZER: ZIP file

The predicted Blumeria graminis f. sp. hordei secretome used in the evaluation: FASTA file
The predicted Blumeria graminis f. sp. hordei EffectorP candidates from the secretome: FASTA file
The predicted Blumeria graminis f. sp. hordei EffectorP candidates from the secretome run through LOCALIZER: ZIP file

The predicted Blumeria graminis f. sp. tritici secretome used in the evaluation: FASTA file
The predicted Blumeria graminis f. sp. tritici EffectorP candidates from the secretome: FASTA file
The predicted Blumeria graminis f. sp. tritici EffectorP candidates from the secretome run through LOCALIZER: ZIP file

The predicted Botrytis cinerea secretome used in the evaluation: FASTA file
The predicted Botrytis cinerea EffectorP candidates from the secretome: FASTA file
The predicted Botrytis cinerea EffectorP candidates from the secretome run through LOCALIZER: ZIP file

The predicted Cladosporium fulvum secretome used in the evaluation: FASTA file
The predicted Cladosporium fulvum EffectorP candidates from the secretome: FASTA file
The predicted Cladosporium fulvum EffectorP candidates from the secretome run through LOCALIZER: ZIP file

The predicted Colletotrichum higginsianum secretome used in the evaluation: FASTA file
The predicted Colletotrichum higginsianum EffectorP candidates from the secretome: FASTA file
The predicted Colletotrichum higginsianum EffectorP candidates from the secretome run through LOCALIZER: ZIP file

The predicted Coniophora puteana secretome used in the evaluation: FASTA file
The predicted Coniophora puteana EffectorP candidates from the secretome: FASTA file
The predicted Coniophora puteana EffectorP candidates from the secretome run through LOCALIZER: ZIP file

The predicted Coprinus cinereus secretome used in the evaluation: FASTA file
The predicted Coprinus cinereus EffectorP candidates from the secretome: FASTA file
The predicted Coprinus cinereus EffectorP candidates from the secretome run through LOCALIZER: ZIP file

The predicted Dacryopinax sp. secretome used in the evaluation: FASTA file
The predicted Dacryopinax sp. EffectorP candidates from the secretome: FASTA file
The predicted Dacryopinax sp. EffectorP candidates from the secretome run through LOCALIZER: ZIP file

The predicted Dichomitus squalens secretome used in the evaluation: FASTA file
The predicted Dichomitus squalens EffectorP candidates from the secretome: FASTA file
The predicted Dichomitus squalens EffectorP candidates from the secretome run through LOCALIZER: ZIP file

The predicted Fomitiporia mediterranea secretome used in the evaluation: FASTA file
The predicted Fomitiporia mediterranea EffectorP candidates from the secretome: FASTA file
The predicted Fomitiporia mediterranea EffectorP candidates from the secretome run through LOCALIZER: ZIP file

The predicted Fomitopsis pinicola secretome used in the evaluation: FASTA file
The predicted Fomitopsis pinicola EffectorP candidates from the secretome: FASTA file
The predicted Fomitopsis pinicola EffectorP candidates from the secretome run through LOCALIZER: ZIP file

The predicted Fusarium graminearum secretome used in the evaluation: FASTA file
The predicted Fusarium graminearum EffectorP candidates from the secretome: FASTA file
The predicted Fusarium graminearum EffectorP candidates from the secretome run through LOCALIZER: ZIP file

The predicted Fusarium oxysporum 47 secretome used in the evaluation: FASTA file
The predicted Fusarium oxysporum 47 EffectorP candidates from the secretome: FASTA file
The predicted Fusarium oxysporum 47 EffectorP candidates from the secretome run through LOCALIZER: ZIP file

The predicted Fusarium oxysporum f. sp. lycopersici secretome used in the evaluation: FASTA file
The predicted Fusarium oxysporum f. sp. lycopersici EffectorP candidates from the secretome: FASTA file
The predicted Fusarium oxysporum f. sp. lycopersici EffectorP candidates from the secretome run through LOCALIZER: ZIP file

The predicted Gloeophyllum trabeum secretome used in the evaluation: FASTA file
The predicted Gloeophyllum trabeum EffectorP candidates from the secretome: FASTA file
The predicted Gloeophyllum trabeum EffectorP candidates from the secretome run through LOCALIZER: ZIP file

The predicted Hebeloma cylindrosporum secretome used in the evaluation: FASTA file
The predicted Hebeloma cylindrosporum EffectorP candidates from the secretome: FASTA file
The predicted Hebeloma cylindrosporum EffectorP candidates from the secretome run through LOCALIZER: ZIP file

The predicted Hysterium pulicare secretome used in the evaluation: FASTA file
The predicted Hysterium pulicare EffectorP candidates from the secretome: FASTA file
The predicted Hysterium pulicare EffectorP candidates from the secretome run through LOCALIZER: ZIP file

The predicted Laccaria amethystina secretome used in the evaluation: FASTA file
The predicted Laccaria amethystina EffectorP candidates from the secretome: FASTA file
The predicted Laccaria amethystina EffectorP candidates from the secretome run through LOCALIZER: ZIP file

The predicted Laccaria bicolor secretome used in the evaluation: FASTA file
The predicted Laccaria bicolor EffectorP candidates from the secretome: FASTA file
The predicted Laccaria bicolor EffectorP candidates from the secretome run through LOCALIZER: ZIP file

The predicted Leptosphaeria maculans secretome used in the evaluation: FASTA file
The predicted Leptosphaeria maculans EffectorP candidates from the secretome: FASTA file
The predicted Leptosphaeria maculans EffectorP candidates from the secretome run through LOCALIZER: ZIP file

The predicted Magnaporthe oryzae secretome used in the evaluation: FASTA file
The predicted Magnaporthe oryzae EffectorP candidates from the secretome: FASTA file
The predicted Magnaporthe oryzae EffectorP candidates from the secretome run through LOCALIZER: ZIP file

The predicted Melampsora laricis-populina secretome used in the evaluation: FASTA file
The predicted Melampsora laricis-populina EffectorP candidates from the secretome: FASTA file
The predicted Melampsora laricis-populina EffectorP candidates from the secretome run through LOCALIZER: ZIP file

The predicted Melampsora lini secretome used in the evaluation: FASTA file
The predicted Melampsora lini EffectorP candidates from the secretome: FASTA file
The predicted Melampsora lini EffectorP candidates from the secretome run through LOCALIZER: ZIP file

The predicted Neurospora crassa secretome used in the evaluation: FASTA file
The predicted Neurospora crassa EffectorP candidates from the secretome: FASTA file
The predicted Neurospora crassa EffectorP candidates from the secretome run through LOCALIZER: ZIP file

The predicted Paxillus involutus secretome used in the evaluation: FASTA file
The predicted Paxillus involutus EffectorP candidates from the secretome: FASTA file
The predicted Paxillus involutus EffectorP candidates from the secretome run through LOCALIZER: ZIP file

The predicted Paxillus rubicundulus secretome used in the evaluation: FASTA file
The predicted Paxillus rubicundulus EffectorP candidates from the secretome: FASTA file
The predicted Paxillus rubicundulus EffectorP candidates from the secretome run through LOCALIZER: ZIP file

The predicted Penicillium digitatum secretome used in the evaluation: FASTA file
The predicted Penicillium digitatum EffectorP candidates from the secretome: FASTA file
The predicted Penicillium digitatum EffectorP candidates from the secretome run through LOCALIZER: ZIP file

The predicted Pichia stipitis secretome used in the evaluation: FASTA file
The predicted Pichia stipitis EffectorP candidates from the secretome: FASTA file
The predicted Pichia stipitis EffectorP candidates from the secretome run through LOCALIZER: ZIP file

The predicted Piloderma croceum secretome used in the evaluation: FASTA file
The predicted Piloderma croceum EffectorP candidates from the secretome: FASTA file
The predicted Piloderma croceum EffectorP candidates from the secretome run through LOCALIZER: ZIP file

The predicted Pisolithus microcarpus secretome used in the evaluation: FASTA file
The predicted Pisolithus microcarpus EffectorP candidates from the secretome: FASTA file
The predicted Pisolithus microcarpus EffectorP candidates from the secretome run through LOCALIZER: ZIP file

The predicted Pisolithus tinctorius secretome used in the evaluation: FASTA file
The predicted Pisolithus tinctorius EffectorP candidates from the secretome: FASTA file
The predicted Pisolithus tinctorius EffectorP candidates from the secretome run through LOCALIZER: ZIP file

The predicted Puccinia graminis f. sp. tritici secretome used in the evaluation: FASTA file
The predicted Puccinia graminis f. sp. tritici EffectorP candidates from the secretome: FASTA file
The predicted Puccinia graminis f. sp. tritici EffectorP candidates from the secretome run through LOCALIZER: ZIP file

The predicted Puccinia striiformis f. sp. tritici PST-130 secretome used in the evaluation: FASTA file
The predicted Puccinia striiformis f. sp. tritici PST-130 EffectorP candidates from the secretome: FASTA file
The predicted Puccinia striiformis f. sp. tritici PST-130 EffectorP candidates from the secretome run through LOCALIZER: ZIP file

The predicted Puccinia triticina secretome used in the evaluation: FASTA file
The predicted Puccinia triticina EffectorP candidates from the secretome: FASTA file
The predicted Puccinia triticina EffectorP candidates from the secretome run through LOCALIZER: ZIP file

The predicted Punctularia strigosozonata secretome used in the evaluation: FASTA file
The predicted Punctularia strigosozonata EffectorP candidates from the secretome: FASTA file
The predicted Punctularia strigosozonata EffectorP candidates from the secretome run through LOCALIZER: ZIP file

The predicted Pyrenophora tritici-repentis secretome used in the evaluation: FASTA file
The predicted Pyrenophora tritici-repentis EffectorP candidates from the secretome: FASTA file
The predicted Pyrenophora tritici-repentis EffectorP candidates from the secretome run through LOCALIZER: ZIP file

The predicted Rhizoctonia solani AG8 secretome used in the evaluation: FASTA file
The predicted Rhizoctonia solani AG8 EffectorP candidates from the secretome: FASTA file
The predicted Rhizoctonia solani AG8 EffectorP candidates from the secretome run through LOCALIZER: ZIP file

The predicted Rhodosporidium toruloides secretome used in the evaluation: FASTA file
The predicted Rhodosporidium toruloides EffectorP candidates from the secretome: FASTA file
The predicted Rhodosporidium toruloides EffectorP candidates from the secretome run through LOCALIZER: ZIP file

The predicted Rhodotorula graminis secretome used in the evaluation: FASTA file
The predicted Rhodotorula graminis EffectorP candidates from the secretome: FASTA file
The predicted Rhodotorula graminis EffectorP candidates from the secretome run through LOCALIZER: ZIP file

The predicted Saccharomyces cerevisiae secretome used in the evaluation: FASTA file
The predicted Saccharomyces cerevisiae EffectorP candidates from the secretome: FASTA file
The predicted Saccharomyces cerevisiae EffectorP candidates from the secretome run through LOCALIZER: ZIP file

The predicted Scleroderma citrinum secretome used in the evaluation: FASTA file
The predicted Scleroderma citrinum EffectorP candidates from the secretome: FASTA file
The predicted Scleroderma citrinum EffectorP candidates from the secretome run through LOCALIZER: ZIP file

The predicted Sclerotinia sclerotiorum secretome used in the evaluation: FASTA file
The predicted Sclerotinia sclerotiorum EffectorP candidates from the secretome: FASTA file
The predicted Sclerotinia sclerotiorum EffectorP candidates from the secretome run through LOCALIZER: ZIP file

The predicted Sebacina vermifera secretome used in the evaluation: FASTA file
The predicted Sebacina vermifera EffectorP candidates from the secretome: FASTA file
The predicted Sebacina vermifera EffectorP candidates from the secretome run through LOCALIZER: ZIP file

The predicted Stagonospora nodorum secretome used in the evaluation: FASTA file
The predicted Stagonospora nodorum EffectorP candidates from the secretome: FASTA file
The predicted Stagonospora nodorum EffectorP candidates from the secretome run through LOCALIZER: ZIP file

The predicted Stereum hirsutum secretome used in the evaluation: FASTA file
The predicted Stereum hirsutum EffectorP candidates from the secretome: FASTA file
The predicted Stereum hirsutum EffectorP candidates from the secretome run through LOCALIZER: ZIP file

The predicted Suillus luteus secretome used in the evaluation: FASTA file
The predicted Suillus luteus EffectorP candidates from the secretome: FASTA file
The predicted Suillus luteus EffectorP candidates from the secretome run through LOCALIZER: ZIP file

The predicted Taphrina deformans secretome used in the evaluation: FASTA file
The predicted Taphrina deformans EffectorP candidates from the secretome: FASTA file
The predicted Taphrina deformans EffectorP candidates from the secretome run through LOCALIZER: ZIP file

The predicted Trametes versicolor secretome used in the evaluation: FASTA file
The predicted Trametes versicolor EffectorP candidates from the secretome: FASTA file
The predicted Trametes versicolor EffectorP candidates from the secretome run through LOCALIZER: ZIP file

The predicted Tremella mesenterica secretome used in the evaluation: FASTA file
The predicted Tremella mesenterica EffectorP candidates from the secretome: FASTA file
The predicted Tremella mesenterica EffectorP candidates from the secretome run through LOCALIZER: ZIP file

The predicted Tulasnella calospora secretome used in the evaluation: FASTA file
The predicted Tulasnella calospora EffectorP candidates from the secretome: FASTA file
The predicted Tulasnella calospora EffectorP candidates from the secretome run through LOCALIZER: ZIP file

The predicted Ustilago maydis secretome used in the evaluation: FASTA file
The predicted Ustilago maydis EffectorP candidates from the secretome: FASTA file
The predicted Ustilago maydis EffectorP candidates from the secretome run through LOCALIZER: ZIP file

The predicted Verticillium dahliae secretome used in the evaluation: FASTA file
The predicted Verticillium dahliae EffectorP candidates from the secretome: FASTA file
The predicted Verticillium dahliae EffectorP candidates from the secretome run through LOCALIZER: ZIP file

The predicted Wolfiporia cocos secretome used in the evaluation: FASTA file
The predicted Wolfiporia cocos EffectorP candidates from the secretome: FASTA file
The predicted Wolfiporia cocos EffectorP candidates from the secretome run through LOCALIZER: ZIP file

The predicted Zymoseptoria tritici secretome used in the evaluation: FASTA file
The predicted Zymoseptoria tritici EffectorP candidates from the secretome: FASTA file
The predicted Zymoseptoria tritici EffectorP candidates from the secretome run through LOCALIZER: ZIP file


Contact

For comments and suggestions please contact jana.sperschneider@csiro.au